3xere tata luc  (Addgene inc)

Journal: Cancer medicine

Article Title: Alternative promoters and splicing create multiple functionally distinct isoforms of oestrogen receptor alpha in breast cancer and healthy tissues.

doi: 10.1002/cam4.6508

Figure Lengend Snippet: FIGURE 3 (A) Schematic illustration of the domain structure of the selected isoforms. (B) Isoform transcription factor activity on a dual-luciferase reporter with firefly luciferase controlled by a 3xERE-containing promoter. Luminescence from firefly luciferase was normalised to Renilla luciferase, and the activity was expressed relative to the baseline value for the empty pEGFP-C1 vector with vehicle. ERfl = full-length oestrogen receptor alpha (ER), vehicle control = 0.1% ethanol, E2 = oestradiol and 4OHT = 4-hydroxytamoxifen. Bars indicate the mean of n = 3 replicates ± standard deviation. Two-tailed Student's t-test was used to test for significant differences in relative luciferase activity between each isoform and ERfl matched by treatment. *p < 0.05, **p < 0.01, ***p < 0.001, and ns = non-significant.

Article Snippet: The pmirGLO- 3xERE reporter was constructed from the 3xERE- containing region of the 3xERE- TATA- luc plasmid25 (Addgene) cloned in the pmirGLO vector (Promega) that had been engineered to introduce a multiple cloning site upstream of the transcription start site.

Techniques: Activity Assay, Luciferase, Plasmid Preparation, Control, Standard Deviation, Two Tailed Test